Journal: PLoS Pathogens

Article Title: Functionally Redundant RXLR Effectors from Phytophthora infestans Act at Different Steps to Suppress Early flg22-Triggered Immunity

doi: 10.1371/journal.ppat.1004057

Figure Lengend Snippet: ( A ) Confocal microscopy of A. thaliana protoplasts expressing GFP-SFI1 or myr-GFP-SFI1 12 h after transfection. Representative optical sections of bright field and merged GFP (in green) and chloroplast (in blue) fluorescence images are shown. ( B ) Representative confocal microscope images of N. benthamiana cells expressing GFP-SFI1 and myr-GFP-SFI1 (left panels, in green) with the nucleolar marker RFP-fibrillarin (right panels, in red); the merged images are shown in the central panels. ( C ) Measurement of pFRK1-Luc reporter activity in A. thaliana protoplasts 6 h after flg22 treatment in the presence of GFP (control), AvrPto, GFP-SFI1 or myr-GFP-SFI1. Pooled data from four experiments are presented as mean ± SEM. Significant differences (p<0.05) in luciferase activity (denoted *) were determined using one-way ANOVA followed by Dunnett's multiple comparison test. ( D, E ) Effect of transient expression of GFP-SFI1 and myr-GFP-SFI1 on P. infestans colonization of N. benthamiana . Mean lesion diameter ( D ) and typical disease symptoms ( E ) are shown for P. infestans 7 days post-inoculation over sites on leaves where an effector construct was agro-infiltrated 1 day earlier. * represents statistical significance (p<0.001) using one-way ANOVA. ( F ) Immunoblot using anti-GFP antibody showing that both GFP-SFI1 and myr-GFP-SFI1 are stable and intact fusion proteins (arrowed) in planta .

Article Snippet: The expression of GFP-tagged PiRXLR effectors was assessed by immunoblotting using polyclonal anti-GFP antibody produced in rabbit or in goat (Acris Antibodies) at a 1/3000 dilution in 5% BSA in TBS-T. For this, protoplast samples were collected 12 (for S. lycopersicum ) or 24 h (for A. thaliana ) after transfection and SDS-PAGE and immunoblotting were carried out as described above.

Techniques: Confocal Microscopy, Expressing, Transfection, Fluorescence, Microscopy, Marker, Activity Assay, Luciferase, Construct, Western Blot

Journal: iScience

Article Title: Zebrafish functional xenograft vasculature platform identifies PF-502 as a durable vasculature normalization drug

doi: 10.1016/j.isci.2023.107734

Figure Lengend Snippet:

Article Snippet: GFP Rabbit pAb , HUABIO , Cat# ET1604-25; RRID: AB_2942066.

Techniques: Recombinant, CCK-8 Assay, Protease Inhibitor, Lysis, Software